Antigenic difference between polar montrichous and peritrichous flagella of Vibrio parahaemolyticus.
نویسندگان
چکیده
Polar monotrichous and peritrichous flagella of Vibrio parahaemolyticus were isolated and purified separately. On hydroxylapatite column chromatography, the flagellins of polar monotrichous flagella were eluted with a higher concentration of phosphate than those of peritrichous flagella. Gel diffusion tests showed an antigenic difference between the flagellins of polar monotrichous and peritrichous flagella. Electron microscope observations on cells stained with ferritin-conjugated antibodies demonstrated that polar monotrichous and peritrichous flagella reacted specifically with antimonotrichous flagellin and antiperitrichous flagellin antisera, respectively.
منابع مشابه
Antigenic Difference Between Polar Monotrichous and Peritrichous Flagella of Vibrio parahaemolyticus
Vibrio parahaemolyticus has peritrichous flagella in addition to polar monotrichous flagella (1, 3, 4, 16, 17; E. Yabuuchi, M. Arita, Y. Takeda, and T. Miwatani, Jap. J. Microbiol., in press), and some differences in the characteristics of these two types of flagella have been reported. Polar monotrichous flagella are a normal type, whereas peritrichous flagella are an atypical, curly type (16;...
متن کاملAnalysis of the polar flagellar gene system of Vibrio parahaemolyticus.
Vibrio parahaemolyticus has dual flagellar systems adapted for locomotion under different circumstances. A single, sheathed polar flagellum propels the swimmer cell in liquid environments. Numerous unsheathed lateral flagella move the swarmer cell over surfaces. The polar flagellum is produced continuously, whereas the synthesis of lateral flagella is induced under conditions that impede the fu...
متن کاملPolar flagellar motility of the Vibrionaceae.
Polar flagella of Vibrio species can rotate at speeds as high as 100,000 rpm and effectively propel the bacteria in liquid as fast as 60 microm/s. The sodium motive force powers rotation of the filament, which acts as a propeller. The filament is complex, composed of multiple subunits, and sheathed by an extension of the cell outer membrane. The regulatory circuitry controlling expression of th...
متن کاملPolar flagella rotation in Vibrio parahaemolyticus confers resistance to bacteriophage infection
Bacteriophage has been recognized as a novel approach to treat bacterial infectious diseases. However, phage resistance may reduce the efficacy of phage therapy. Here, we described a mechanism of bacterial resistance to phage infections. In Gram-negative enteric pathogen Vibrio parahaemolyticus, we found that polar flagella can reduce the phage infectivity. Deletion of polar flagella, but not t...
متن کاملLateral flagellar gene system of Vibrio parahaemolyticus.
Vibrio parahaemolyticus possesses dual flagellar systems adapted for movement under different circumstances. A single polar flagellum propels the bacterium in liquid (i.e., swimming) with a motor that is powered by the sodium motive force. Multiple proton-driven lateral flagella enable translocation over surfaces (i.e., swarming). The polar flagellum is produced continuously, while production o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of bacteriology
دوره 120 2 شماره
صفحات -
تاریخ انتشار 1974